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Ready the Blotter
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- After all the transfer stacks have been assembled, add the lid by sliding the 3 guides into
the 3 grooves on the base. Add a weight (no more than1 kg) on the cover to ensure even contact
between the electrodes and transfer stacks.
- Make sure the power supply is off and that both the current and voltage controls are set to
zero. If possible, set the voltage limit to 50 V.
- Then connect the leads between the cover and base. Adjust the current to 75 mA (the current
should not exceed 0.8 mA/cm2 of gel). The transfer will take 30 minutes.
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