CSI Logo
Dedicated to the long-standing tradition of Systems Biology
ArrowSearch ArrowUW Directories
ArrowCSIArrowEducationArrowEducation Lab ReferenceArrowBallard HS ProtocolsArrowPolymerase Chain ReactionArrowPCR Protocol
Top Curve
Side Curve
Polymerase Chain Reaction

White Dot
PCR Overview

Master Mix Steps

PCR Buffer

Primers for PCR

Primer Lengths

MgCl2

dNTPs

Taq Polymerase

PCR Steps

Add Master Mix

Thermocycler Overview

Denature

Anneal

Extend

Repeat

PCR Protocol Overview
Materials:
  1. Master Mix
  2. Mineral Oil
  3. DNA samples
Steps:
  1. Add Mineral Oil to tube
  2. Add Master Mix
  3. Add DNA sample
  4. Load in Thermocycler and program
  5. Remove
  6. Store
PCR TUTORIAL
Click to view movie

RELATED READING

Bottom UW/CSI Logo Copyright 2002, CSI
Department to Bioengineering University of Washington