PCR Overview

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Polymerase Chain Reaction

Overview


	Polymerase Chain Reaction PCR Overview Master Mix Steps PCR Buffer Primers for PCR Primer Lengths MgCl₂ dNTPs Taq Polymerase PCR Steps Add Master Mix Thermocycler Overview Denature Anneal Extend Repeat

PCR Overview

Polymerase Chain Reaction allows us to make many copies of a selected fragment of DNA, using 1) special enzymes, 2) the basic building blocks of DNA, and 3) a cycle of heating and cooling to instigate the reaction.