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Polymerase Chain Reaction

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PCR Overview

Master Mix Steps

PCR Buffer

Primers for PCR

Primer Lengths

MgCl2

dNTPs

Taq Polymerase

PCR Steps

Add Master Mix

Thermocycler Overview

Denature

Anneal

Extend

Repeat

Master Mix Protocol
The PCR Master Mix contains everything you need to successfully copy your selected fragment of DNA.


Materials
  1. TBE Buffer
  2. Primers (forward and reverse)
  3. MgCl2
  4. dNTP
  5. dH2O
  6. Taq Polymerase

Steps
  1. Measure and mix
  2. Add polymerase last

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