MgCl2

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Ballard HS Protocols

Polymerase Chain Reaction

MgCl₂


	Polymerase Chain Reaction PCR Overview Master Mix Steps PCR Buffer Primers for PCR Primer Lengths MgCl₂ dNTPs Taq Polymerase PCR Steps Add Master Mix Thermocycler Overview Denature Anneal Extend Repeat

MgCl₂

Magnesium Chloride is required for Taq Polymerase to function effectively. When the enzyme polymerase binds to the DNA strand, it requires magnesium ions with hydroxide groups to remove a hydrogen proton from the deoxyribose of the nucleotide, in order to add the next nucleotide.

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