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Ballard HS Protocols
Polymerase Chain Reaction
Thermocycler Overview
Polymerase Chain Reaction
PCR Overview
Master Mix Steps
PCR Buffer
Primers for PCR
Primer Lengths
MgCl
2
dNTPs
Taq Polymerase
PCR Steps
Add Master Mix
Thermocycler Overview
Denature
Anneal
Extend
Repeat
Thermocycler Overview
The thermalcycler does the repetitive job of bringing your samples to the right temperature for the right length of time and then repeating as many times as you tell it to do so.
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