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Polymerase Chain Reaction

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PCR Overview

Master Mix Steps

PCR Buffer

Primers for PCR

Primer Lengths

MgCl2

dNTPs

Taq Polymerase

PCR Steps

Add Master Mix

Thermocycler Overview

Denature

Anneal

Extend

Repeat

Thermocycler Overview


The thermalcycler does the repetitive job of bringing your samples to the right temperature for the right length of time and then repeating as many times as you tell it to do so.
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