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Ballard HS Protocols
PCR
Ballard HS Protocols
PCR Overview
Master Mix Steps
PCR Buffer
Primers for PCR
Primer Lengths
MgCl
2
dNTPs
Taq Polymerase
PCR Steps
Add Master Mix
Thermocycler Overview
Denature
Anneal
Extend
Repeat
Polymerase Chain Reaction
PCR Overview
Master Mix Steps
TBE Buffer
Primers (Forward and Reverse)
The Importance of Primer Length
MgCl
2
dNTP
Taq Polymerase
PCR Steps
Add Master Mix
Load in Thermocycler and program
Denaturation
Annealing
Extending
Repeating