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Polymerase Chain Reaction

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PCR Overview

Master Mix Steps

PCR Buffer

Primers for PCR

Primer Lengths

MgCl2

dNTPs

Taq Polymerase

PCR Steps

Add Master Mix

Thermocycler Overview

Denature

Anneal

Extend

Repeat

Denature
Heat is used to break the bonds that hold the two strands of double-stranded DNA together to form the double helix. The temperature (95°C) is high enough to "melt" the hydrogen bonds.
DENATURE TUTORIAL
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Department to Bioengineering University of Washington