PCR Buffer

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Polymerase Chain Reaction

PCR Buffer


	Polymerase Chain Reaction PCR Overview Master Mix Steps PCR Buffer Primers for PCR Primer Lengths MgCl₂ dNTPs Taq Polymerase PCR Steps Add Master Mix Thermocycler Overview Denature Anneal Extend Repeat

PCR Buffer

Buffers are solutions designed to maintain the best pH balance (acid or base) for your reaction, in this case PCR. The standard PCR buffer is Tris plus potassium chloride (KCl₂). Tris is short for trishydroxymethylaminomethane

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