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Stain with Ethidium Bromide
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This procedure will be done by your teacher. (see below) Ethidium Bromide
(EtBr) binds to DNA and fluoresces under ultraviolet light. When your gel is
stained with EtBr, the EtBr will attach to the DNA molecules in your samples.
Later, when you place your sample under ultraviolet light, the EtBr will show
you where your DNA molecules are located. If the PCR procedure worked, you
will have visible bands on the gel.
Caution: EtBr is a
known mutagen. It causes mutations. It will adhere to your DNA just as it
will adhere to the DNA on your gel. Always wear gloves when handling your
gel.
Teacher
happily handles known mutagen to protect students. |
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